Journal: Applied and Environmental Microbiology

Article Title: Biosynthesis of Fusarubins Accounts for Pigmentation of Fusarium fujikuroi Perithecia

doi: 10.1128/AEM.00823-12

Figure Lengend Snippet: Information about the putative fsr gene cluster

Article Snippet: Phylogenetic analysis was performed by comparing the KS domains using the Web-based tool at www.phylogeny.fr ( 23 ). table ft1 table-wrap mode="anchored" t5 caption a7 Gene Length (bp) Intron No. of amino acids InterPro annotation Domains and motifs Predicted function FF _ 03983 1,788 0 595 IPR002937 Amine oxidase Unknown function fsr1 ( FF _ 03984 ) 7,069 4 2,286 IPR014031 β-Ketoacyl synthase Nonreducing PKS responsible for the condensation of seven acetyl-CoA units IPR014043 Acyltransferase domain IPR009081 Acyl carrier protein-like IPR013120 Thioesterreductase fsr2 ( FF _ 03985 ) 1,209 1 387 IPR001077 O -Methyltransferase, family 2 Methyltransferase responsible for the methylation of hydroxyl groups at C-6 and C-8 fsr3 ( FF _ 03986 ) 1,660 2 522 IPR002938 Monooxygenase, FAD binding Monooxygenase responsible for reduction/oxidation of the PKS-derived aldehyde as well as for incorporation of hydroxyl groups at C-5, C-10, and C-13 IPR003042 Aromatic-ring hydroxylase-like fsr4 ( FF _ 03987 ) 1,117 2 338 IPR002085 Alcohol dehydrogenase superfamily, zinc containing Unknown function IPR016040 NAD(P)-binding domain fsr5 ( FF _ 03988 ) 794 1 256 IPR002198 Short-chain dehydrogenase/reductase Unknown function fsr6 ( FF _ 03989 ) 1,014 0 338 IPR001138 Zn(II)2C6 fungal-type DNA-binding domain Zn(II)2Cys6 transcription factor IPR013700 Aflatoxin-regulatory protein FF _ 03990 1,181 1 374 None Unknown function Open in a separate window Information about the putative fsr gene cluster Plasmid constructions.

Techniques: Methylation, Binding Assay